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A competitive indirect time-resolved fluoroimmunoassay (TRFIA) was developed for detection of zearalenone (ZEN) in cereals, in which ZEN conjugated to bovine serum albumin (BSA) is used as solid-phase antigen. A competitive indirect TRFIA was conducted by simultaneously incubating ZEN in standard or extracted samples with anti-ZEN monoclonal antibody over ZEN-BSA coated plates, and then determining...
Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen I (PGI) with time-resolved flu-oroimmunoassay (TRFIA) as a detection technique. On the noncompetitive assay, captured monoclonal antibodies (McAbs) coated on wells were directed against a specific antigenic site on the PGI. Another McAbs, called as labeling McAbs, were prepared with the Eu-chelate of N-(p-isothiocyanatobenzyl)-diethylenetriamine-N,...
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