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This paper presents a multicellular tumor spheroid formation and extraction chip using removable cell trapping barriers. The previous spheroid formation chips using fixed cell trapping barriers have difficulties in extracting the formed spheroids. The present spheroid chip, however, achieves simple spheroid extraction using the removable cell trapping barriers formed by membrane inflation. A 4 by...
We present a digital dilution chip, capable of exponential sample dilution and reaction of them with another sample, using the selective control of inter-well valves. The present chip discretely merges and splits a fixed fluidic volume in the well array by controlling inter-well valves around a well. Thus, it is easy to achieve a fixed volume of the diluted samples and make reaction of them with another...
We present passive flow-rate regulators using parallel membrane valves rectifying dynamic inlet pressure. The parallel membrane valves in the passive flow-rate regulator are designed to deflect and adjust the flow resistance according to the inlet pressure, thus maintaining a constant flow-rate independent of the inlet pressure variation. The prototype was fabricated by PolyDiMethylSiloxane (PDMS)...
We present a novel cell-deformability-monitoring chip based on the digitally measured cell-lysis rate which is dependent on the areal strain of the cell membrane. This method offers simple and automated cell-deformability monitoring based on the mechanical property of the cell membrane. We suggest a filter design that considers the areal strain imposed on the cell membrane passing through the filter...
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