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Two α-galactosidases, AgaAJB07 from Mesorhizobium and AgaAHJG4 from Streptomyces, were expressed in Escherichia coli. Recombinant AgaAJB07 showed a 2.9-fold and 22.6-fold increase in kcat with a concomitant increase of 2.3-fold and 16.3-fold in Km in the presence of 0.5mM ZnSO4 and 30.0mM Pb(CH3COO)2, respectively. Recombinant AgaAHJG4 showed apparent optimal activity at pH 8.0 in McIlvaine or Tris–HCl...
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