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Digital micro fluidic biochips with non-regular arrays are of interest for clinical diagnostic applications in a cost-sensitive market segment. Previous techniques for biochip testing are limited to regular micro fluidic arrays. We present an automatic test pattern generation (ATPG) method for non-regular digital micro fluidic chips. The ATPG method can generate test patterns to detect catastrophic...
Microfluidics-based biochips are revolutionizing high-throughput sequencing, parallel immunoassays, blood chemistry for clinical diagnostics, and drug discovery. These devices enable the precise control of nanoliter volumes of biochemical samples and regents. They combine electronics with biology, and they integrate various bioassay operations, such as sample preparation, analysis, separation, and...
Digital microfluidic biochips are being utilized in many areas of biochemistry and biomedical sciences. Since cross-contamination between droplets of different biomolecules can lead to erroneous outcomes for bioassays, it is essential to avoid cross-contamination during droplet routing. We propose a wash-operation synchronization method to manipulate wash droplets to clean the residue that is left...
Widely building of the large span underground water seal oil caverns must gradually propose more stringent requirements of the design level of oil caverns. The large span underground water seal oil cavern not only needs to rely on the natural conditions of the buried groundwater, but must also relies on the water curtain hole installed around the cavern in a certain pressure to provide a stable water...
The multiplexed in-vitro measurement of glucose and other metabolites in human physiological fluids is of great importance for the clinical diagnosis of metabolic disorders. We present a design technique for optimizing an n-plex bioassay on an electrowetting-based digital microfluidic lab-on-chip. The n product droplets of the n-plex bioassay are detected using a photomultiplier tube located at the...
Recent advances in droplet-based digital microfluidics have enabled biochip devices for DNA sequencing, immunoassays, clinical chemistry, and protein crystallization. Since cross-contamination between droplets of different biomolecules can lead to erroneous outcomes for bioassays, the avoidance of cross-contamination during droplet routing is a key design challenge for biochips. We propose a droplet-routing...
Dependability is an important system attribute for microfluidic lab-on-chip. Structural test and functional test are needed to detect defects and malfunctions, respectively. Previously proposed techniques for reading test outcomes and for pulse-sequence analysis are cumbersome and error-prone. We present a built-in self-test (BIST) method for digital microfluidic lab-on-chip. This method utilizes...
Dependability is an important system attribute for microfluidic lab-on-chip devices. On-line testing offers a promising method for detecting defects, fluidic abnormalities, and bioassay malfunctions during chip operation. However, previous techniques for reading test outcomes and analyzing pulse sequences are cumbersome, sensitive to the calibration of capacitive sensors, and error-prone. We present...
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