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Gene edition using the CRISPR/Cas9 system has been achieved in few crustaceans, but in penaeid shrimps, several hurdles exist, restraining the potential of CRISPR‐Cas9 technology in shrimp Aquaculture. A key step for gene editing is the efficient introduction of the CRISPR‐Cas9 system into the cells. Although microinjection is an effective method for CRISPR‐Cas9 system delivery into zygotes, the successful...
Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg−1 of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented...
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