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Evaluation of oocyte and embryo has become important for many biomedicai applications. We developed measurement of oxygen consumption rate of individual oocyte using fluorescence oxygen sensor having stripe-shape on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The...
In this paper, we proposed cell investigation tool for measurement of physiological conditions of single cell. We developed a novel temperature measurement method using temperature dependence of the quantum dot. The cell investigation tool encapsulates the quantum dot and adheres to the cell membrane. These tools can be manipulated in a solution by optical tweezers. We proposed image processing of...
In this paper, we proposed a long-lifetime temperature measurement using color shift of the fluorescence from the quantum dots. The cell investigation tool encapsulates the quantum dots and can adhere to the cell membrane. These tools can be manipulated in a solution by optical tweezers. We used image processing of fluorescent image of the tools to acquire the color information from the fluorescent...
On-chip environment measurement is an important technique for control ambient conditions of cells and monitoring cell activities. However, integration of many sensors in the chip has difficulties such as position and interconnection of sensors. We developed hydrogel microsensors made of photocrosslinkable resin impregnating with indicators for on-chip measurement. This sensor can be used for both...
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