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An UPLC–MS/MS method for determination of ten steroid hormones in animal origin food has been developed with pretreatment of the samples by matrix solid-phase dispersion (MSPD). The MSPD conditions, including the dispersing sorbents, elution solvents, ratio of sorbent to sample and the volume of the elution solvent have been investigated and optimised, and the method has been evaluated and validated...
The interaction mechanism of 3,7-dihydroxyflavone (3,7-diHF) and human serum albumin (HSA) was investigated by fluorescence quenching, fluorescence enhancement, steady-state and time-resolved fluorescence emission and UV–vis absorption spectrometry. The binding site of 3,7-diHF on protein was determined by investigating the spectroscopic properties of 3,7-diHF–HSA complex at pH 7.4 and pH 3.5 individually,...
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