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Using suppression subtractive hybridization technique, a purpose‐designed cDNA library was established to investigate the changes in gene expression and isolate the immune‐related genes induced or activated by live attenuated Vibrio anguillarum. Forty‐eight genes were obtained in flounder immune tissues, including cytokines, cytokine receptors, cell surface molecules, transcription factors, complement...
Vibrio angillarum is an important pathogen for large yellow croaker, being responsible for significant economic losses in Chinese aquaculture. Gene expression was studied in large yellow croaker stimulated with an attenuated live V. anguillarum MVAV6203. Subtractive cDNA libraries were constructed by suppression subtractive hybridization (SSH) from 3 immune-relevant tissues during the process. Approximately...
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