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Studies on the effects of environmental pollutants to protein in vitro has become a global attention. Hydrogen peroxide (H 2 O 2 ) is used as an effective food preservative and bleacher in industrial production. The toxicity of H 2 O 2 to trypsin was investigated by multiple spectroscopic techniques and the molecular docking method at the molecular level. The intrinsic...
The toxicity of hydroxyl group of isopropanol to trypsin in aqueous solution was investigated by techniques including UV–visible absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, enzyme activity assay and molecular docking technology. The results of UV–visible absorption spectroscopy and CD spectra indicate that isopropanol could change the secondary structure...
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