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The pathogenesis of arterial thrombotic disease involves multiple genetic and environmental factors related to atherosclerosis and thrombosis. The endothelium is a monolayer of polygonal cells that extend continuously over the luminal surface of the entire vasculature. Injury to the endothelium leads to dysfunction. The causes of injury include lipids, immune complexes, microorganisms, smoking, hypertension,...
We have investigated the role of human neutral sphingomyelinase (N-SMase) on TNF-α induced apoptosis in Human Leukemic (HL-60) cells. We have employed three diverse methods to assess TNF-α induced apoptosis in these cells, these are: DNA ladder, a hallmark in apoptosis; thymidine release assay; and fluorescence-assisted cell sorting (FACS). We found that TNF-α induced apoptosis in HL-60 cells in...
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