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Rapid and accurate diagnosis of C-reactive protein (CRP) is crucial for monitoring cardiovascular diseases because it is a well-known biomarker for evaluating risks of cardiovascular diseases. This study presents a dual-aptamer assay for detection of CRP by using field-effect transistors (FET). This is the first time that two aptamers, which are specific to CRP, were used to form a sandwich assay...
AlGaN/GaN high electron mobility transistors (HEMTs) were immobilized with various receptors, including antibodies, duplex DNA, and HIV reverse transcriptase (RT) enzymes to detect ligands, including peptides, SARS proteins, and HIV drugs, respectively. Signals generated by the sensors were fitted into binding-site models and analyzed. The dissociation constants of the ligand-receptor pairs and the...
In this article, we used AlGaN/GaN high electron mobility transistors (HEMTs) to construct the first label-free semiconductor-sensor-based binding assay to our knowledge. Our results suggested that the nucleotide-c terminal domain of SARS coronavirus (SARS-CoV) nucleocapsid protein interaction is a two-step binding event with two dissociation constants (Kd1 = 0.052 nM, and Kd2 = 51.24nM) extracted...
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