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The gene encoding a β- galactosidase was cloned from Lactobacillus plantarum FMNP01 and expressed in Escherichia coli BL21(DE3). The characteristics of this purified recombinant enzyme, L.pFMNP01Gal, were determined, and its transgalactosylation reaction conditions for the production of lactulose were optimized. Using ONPG as substrate, the L.pFMNP01Gal showed specific activity of 980U/g with a Km...
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