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Using C4–C25 fatty acid methyl esters (C4–C25 FAMEs) as a sample reference series, a method was developed to generalize the reference system for calculating the second dimension retention index (2I) of compounds analyzed by comprehensive two-dimensional gas chromatography–mass spectrometry (GC × GC–MS). The second dimension elution temperature (2Te), second dimension unadjusted retention time (2tR...
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