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A gene coding for lipase (Tm1350) from the hyperthermophilic bacterium Thermotoga maritima MSB8 was cloned and overexpressed by Escherichia coli . The enzyme can degrade substrates with both short and long acyl chain lengths. The apparent Km and Vmax values for p -nitrophenyl butyrate were 8 mM and 333 U/mg, respectively. The enzyme displayed optimal activity...
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