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Ovine herpesvirus-2 (OvHV-2) is the causative agent for sheep-associated malignant catarrhal fever, which has never been propagated in vitro. Previous studies from this laboratory demonstrated significantly high levels of OvHV-2 DNA in sheep nasal secretions, suggesting a likely avenue of transmission. In the present study, real-time PCR was used to identify sheep experiencing an episode of intense...
A recently developed competitive PCR for ovine herpesvirus 2 (OvHV-2) was used to examine the levels of viral DNA in nasal secretions and peripheral blood leukocytes (PBL) of lambs and adult sheep. Viral DNA first appeared in the PBL of most lambs after about 3 months of age and the levels remained relatively constant thereafter. In most of the lambs (83%, n=12), viral DNA was undetectable by PCR...
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