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Glucan sulfates with different degree of substitution were prepared from partially sulphated insoluble β-1, 3-D-glucan isolated from Saccharomyces cerevisiae cell wall. Sulphation was done with sulfate acid (H2SO4) in dimethyl sulfoxide (DMSO) in presence of urea. Varying reaction times, temperature and ratio of H2SO4 and DMSO were used to obtain glucan sulfates with distinct degree of substitution,...
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