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Prim‐O‐glucosylcimifugin, cimifugin, and 5‐O‐methylvisamminoside are three major chromone derivatives of Saposhnikovia divaricata that have many pharmacological activities, such as anti‐inflammatory and antitumor activities. In the present work, an effective method for the simultaneous separation of prim‐O‐glucosylcimifugin, cimifugin, and 5‐O‐methylvisamminoside with high purities was established...