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Lipocalin-type prostaglandin D synthase (L-PGDS) is highly expressed in the adult testis and epididymis of many mammals. The present study was to investigate L-PGDS expression in mouse testis and epididymis during sexual maturation, and the effects of testoster-one replacement on L-PGDS expression in epididymis by in situ hybridization and immunohistochemistry. Both L-PGDS mRNA and protein were highly...
The purpose of this study was to assess sperm quality in extended boar semen during in vitro storage in order to determine which extender should be used and how long boar semen can be stored. Freshly ejaculated boar semen was diluted with equal volumes of Beltsville thaw solution (BTS), Androhep, KIEV or Zorlesco extenders and stored at 17 o C for up to 15 days. Sperm quality was evaluated...
Calcitonin has been shown to be a progesterone-regulated potential marker of the receptive endometrium in the rat and human. The present study was undertaken to immunohistochemically investigate the changes in calcitonin in the monkey uterus during the menstrual cycle and periimplantation period. Calcitonin immunostaining was primarily localized in the glandular epithelium on d 16, 20, and 25 of the...
The purpose of this study was to assess the sperm quality of fresh ejaculated boar semen stored under different temperatures for up to 48 h in order to use the fresh semen efficiently. Spermatozoa were evaluated by 4 methods: Using trypan blue staining, the viability of spermatozoa stored at 39, 20, 15 and 4 o C for 48 h were 1.6, 46.9, 42.0 and 31.0%, respectively. Employing the hypoosmotic...
Purpose/Objective: The major stumbling block to successful gene therapy today is poor gene transfer. We hypothesized that ionizing radiation might activate cellular recombination, and so improve stable gene transfer. We further hypothesized that known DNA-damage-repair proteins might also be important in radiation-activated recombination.Materials and Methods: The effect of irradiation on stable...
Purpose/Objective: We have previously demonstrated that ionizing radiation can activate a DNA recombination pathway in mammalian cells. In this project, we investigated the role of p53 in radiation activated recombination in ovarian tumor cell lines, and also the effect of p53 status on radiation sensitivity in this cell system.Materials & Methods: PA-1 teratocarcinoma cells, which express wild...
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