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We report on an experiment for mapping two- dimensional blood oxygen saturation distribution by measuring multispectral images in the wavelength range from 500 to 650 nm with the resolution of 7 nm. The multispectral images of the retina are acquired with the originally designed imaging system equipped with the tunable spectral filter. To separate retinal blood vessels from other tissue area, morphological...
We have developed a method for measuring the temperature of micro-regions in aqueous solutions using near-infrared spectroscopy that enables us to measure the temperature of biological cells, tissues, and biochemical solutions in vitro. The measurement principle is based on the fact that the peak wavelength of the water absorption band with its center near 1450 nm shifts with changes in temperature...
Two-dimensional hemoglobin oxygen saturation measurement is demonstrated by using the combination technique of multispectral imaging and the polarization control. Multispectral images are acquired at the wavelength range from 500 to 680 nm to observe the wavelength-dependent diffusely reflected light from the skin tissue. For eliminating the superficially reflected light from the skin, the skin tissue...
The advanced imaging technique for visualizing two-dimensional water content distribution in the stratum corneum of human skin is proposed. The method involves two elemental principles of spectral filtering and imaging with polarization control. It is found in the in vivo experiment in the visible and the near-infrared range that the reflectance spectra of skin tissue in the wavelength range shorter...
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