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Using acetylene to inhibit the reduction of N 2 O to N 2 is the simplest and most commonly used technique to quantify denitrification. The acetylene treatment may not always inhibit N 2 O-reductase completely when soils are wet, low in NO -3 or with a high C-to-NO -3 ratio. Acetylene blocks nitrification as well as N 2 O-reductase, so if...
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