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We tested the hypothesis that shedding of membrane FasL is a mechanism for downregulating FasL/Fas signaling and both membrane and soluble FasL are involved in cardiomyocyte hypoxia/ reoxygenation (H/R) injury. We examined the relative importance of mitochondrial damage and direct cleavage of the executioner caspases by activated initiator caspase-8 in the propagation of FasL/Fas signaling activated...
We verify whether P-glycoprotein (P-gp) could induce cell resistance to apoptosis by inhibiting caspase-8 and caspase-3. Human KB cells, either drug-sensitive or with multidrug resistance (MDR) phenotype caused by Over- expression of P-gp (KBv200 cells), were treated with anti-Fas (CH-11) to induce apoptosis. Cytotoxicity was detected by MTT assay. Symptoms of cell death were assessed by morphological...
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