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CHO cells express both of the phosphoinositidase C-linked G-proteins G q and G 11 G 11 α is some 2.5-fold more highly expressed than G q α in membranes of these cells. Following transfection and stable expression of CHO cells with DNA encoding the human muscarinic M1 acetylcholine (HM1) receptor, chronic treatment of the cells with the cholinergic agonist...
The existence of four distinct muscarinic acetylcholine receptor genes (m1 - m4) has recently been demonstrated. cDNAs for three of these receptors have been cloned from brain (m1, m3, m4) and one from heart (m2). To gain some understanding of the physiological role of the brain muscarinic receptors, we mapped the distribution of their mRNAs in rat brain by in situ hybridization. These mRNAs are barely...
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