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A clinical isolate from a patient was identified as Mycobacterium chimaera , a recently identified species of nontuberculous Mycobacteria . The biochemical and molecular identity, drug sensitivity and virulence of this isolated strain were investigated. 16S rRNA, the 16S-23S ITS, hsp65 and rpoB were amplified, and their sequence similarities with...
In the original proposal of Wayne et al. (Int J Syst Bacteriol 37:463–464, 1987), two measures of genetic relatedness were proposed to set the boundary for prokaryotic species. The first was the change in the melting temperature (ΔTm) of heteroduplex DNA and the second was the extent of DNA–DNA hybridization (DDH). While this approach was justified given the experimental error inherent in these methods,...
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