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The radioresistant N10 and parental KB cell lines were examined for the expression of human DNA repair genes which were related to the repair of radiation-induced DNA damage by northern blot analysis using five kinds of DNA probes (XRCC1, XRCC3, XRCC5, RAD51, RAD52). In the unirradiated condition, N10 cells showed higher expression of XRCC1, XRCC3 and RAD51 mRNA than did KB cells. The X-irradiation...
In order to investigate the radioresistance mechanism in human carcinoma cells, we isolated the radioresistant cells from a human KB carcinoma cell line by various methods. Although the radioresistant cells were not isolated by the repeated X-irradiation method, pretreatment of the cells with mutagens including N-methyl-N nitro-N-nitrosoguanidine and 4-nitroquinoline 1-oxide induced radioresistance...
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