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In this paper, we describe a system for tracking multiple fluorescent particles in a confocal microscope. The tracking algorithm relies on position estimates derived from fluorescence measurements taken at a small number of discrete locations combined with an LQG controller. We report experimental results of tracking a single fixed particle, a single diffusing particle, and two fixed particles.
This paper describes a scheme to track multiple fluorescent particles diffusing in two dimensions. Fluorescence intensities are measured using a scanning-stage confocal setup. We develop a combined model for the position of the particles and the dynamics of the piezo-stage and design a linear-quadratic-Gaussian controller to drive the overall estimation error to zero. The scheme is illustrated through...
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