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A total of 169 Escherichia coli strains were isolated from cows with cases of clinical mastitis. β-Glucuronidase production, serum sensitivity, and susceptibility to selected antibacterials were analyzed using the fluorometric β-glucuronidase assay. About 89% (150 to 169) of the isolates tested positive for β-glucuronidase. Of these isolates producing β-glucuronidase, 102 (68%) were resistant or moderately...
The growth of common mastitis-causing bacteria in milk was followed by a fluorometric technique based on the release of fluorescent 4-methylumbelliferone (4-MU) from 4-methylumbelliferyl-β-D-glucuronide by the β-glucuronidase of bacterial or milk origin. Three of four Escherichia coli strains, all four strains of Streptococcus uberis (4) and Streptococcus agalactiae (4) produced β-glucuronidase....
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