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The immobilization of nucleic acids onto substrates in array fabrication is a complex process involving three major steps: (i) the chemical modification of the arrayed material in such a fashion that it can interact with complementary functionalities present on the substrate to form a stable bond; (ii) the coating of the support surface with adequate functional groups to allow specific binding and...
α-Amylase accumulated in the growth medium of the yeast Lipomyces starkeyi (NCYC 1436) to a maximum of 12 units ml−1 after 15 days' batch culture in a soluble starch medium. Extra α-amylase activity, about 50% of that released into the medium, could be extracted from the cells by a salt wash. α-Amylase was purified by salt precipitation followed by ion exchange chromatography and gel filtration. Native...
The detection of genetically modified foodstuffs is becoming both a food sales and legal necessity. This study reports a rapid DNA extraction/PCR-based method for the detection of genetically modified soya (GMS) and maize (GMM) in mixed samples of transgenic and unmodified soybeans and maize kernels, and a variety of processed samples including soya flour, soya protein isolates, extruded defatted...
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