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Nucleic acid samples with high concentrations of salt could be stacked and well separated during capillary zone electrophoresis (CZE) by adding glycerol into the samples and using a Tris-Borate-EDTA (TBE) buffer (pH 8.3) as the separation medium. The so-called glycerol-salt mediated stacking was found applicable to different types of nucleic acids. Three nucleic acids: a 16s rRNA (1,542 nt), a double...
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