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Papain (EC 3.4.22.2) has been chemically modified using two reagents including different anhydrides of maleic and poly-maleic acids. The average ratio of modified-NH2 was tested by trinitrobenzenesulfonic acid (TNBS) method. The native and modified papain were purified by dialysis and their structures were characterized by FTIR. The factor related with the activity of the modified papain, such as...
Protein structure prediction by using bioinformatics can involve sequence similarity searches, multiple sequence alignments, identification and characterization of domains, secondary structure prediction. The secondary and tertiary structure of stearoyl ACP desaturase was predicted by bioinformatics methods from the mRNA sequence. The stearoyl ACP protein, as a member of plant fatty acid synthesis...
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