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A sensitive and selective liquid chromatography–tandem mass spectrometry method for the determination of pethidine and atropine in rabbit plasma was developed and validated. The analytes and internal standard (IS) are extracted from plasma by liquid–liquid extraction using ethyl acetate, and separated on a Zorbax SB-Aq column (2.1 × 150 mm, 3.5 μm) using acetonitrile–0.1% formic acid as mobile phase...
A simple liquid chromatography-electrospray ionization-mass spectrometric (LC–ESI–MS-MS) method was developed for determination of pirfenidone in rat plasma. After addition of phenacetin as internal standard, protein precipitation with 10% perchloric acid was employed for the sample preparation. Chromatographic separation was achieved on a C18 column, with 40: 60 (v/v) acetonitrile-0.1% formic acid...
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