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This study describes, for the first time, the development and validation of a highly selective and sensitive heterogeneous fluoroimmunoassay (FIA) for the bioanalysis of two monoclonal antibodies (mAbs) used for cancer immunotherapy: bevacizumab (BEV) and cetuximab (CET). The assay combines reliable non-competitive binding of BEV and CET to their specific cell receptor proteins (human vascular endothelial...
A new colorimetric and fluorescent chemosensor for visual determination of carbonate ions was developed by the microwave assisted solvent free synthesis of 7,8-dihydroxy-3-(4-methylphenyl) coumarin (DHMC). The structural characterization of DHMC was confirmed by microanalysis and spectroscopy methods (MALDI-TOF, FT-IR, 1H NMR, 13C NMR, and 2D HETCOR). The binding behaviors of DHMC were investigated...
For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au–In2O3 nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au-In2O3-CS/ABPE). The phase structures, composition and morphology of Au–In2O3 nanocomposite were characterized by X-ray diffraction (XRD), energy–dispersed X-ray (EDX) and transmission...
The GO-COOH/AuNPs/ZnAPTPP nanocomposites were constructed using zinc monoamino porphyrin (ZnAPTPP) through π–π conjugation with carboxylated graphene oxide (GO-COOH) loaded with Au nanoparticles (AuNPs). Prepared materials were characterized by 1H NMR spectra, UV–vis absorption spectroscopy and electrochemical impedance spectroscopy. ITO electrode surface was modified with the prepared nanocomposites...
Meso-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin sulfonate (TDMPzP), a water-soluble porphyrin derivative, was synthesized and used as a colorimetric reagent for Cu2+ detection on a microfluidic paper-based analytical device (µPAD) using distance-based quantification. TDMPzP showed a high selectivity for Cu2+ detection in aqueous solutions. When Cu2+ was added to the TDMPzP under acidic conditions,...
In this study, we developed an ingenious yet effective strategy for cysteine detection. The colorimetric cysteine assay is established through an indicator displacement process, where Cu2+ and pyrocatechol violet (PV) was employed as receptor and indicator, respectively. Proof-of-concept trials demonstrated that the stronger binding affinity of Cu2+ receptor toward cysteine than PV indicator endowed...
An ultra-sensitive electrochemical biosensor was successfully developed for rapid detection of pirimiphos-methyl in olive oil, based of genetically-engineered acetylcholinesterase (AChE) immobilization into electrospun chitosan/poly (vinyl alcohol) blend nanofibers. Due to their unique properties such as spatial structure, high porosity, and large surface area, the use of nanofibers allowed improving...
This study describes the synthesis, characterization and application of a new chrysin-based silica core–shell magnetic nanoparticles (Fe3O4@SiO2-N-chrysin) as an adsorbent for the preconcentration of Cu(II) from aqueous environment. The morphology, thermal stability and magnetic property of Fe3O4@SiO2-N-chrysin were analyzed using FTIR, FESEM, TEM, XRD, thermal analysis and VSM. The extraction efficiency...
Elucidation and quantitative determination of some of commonly used penicillins (ampicillin, penicillin G and carbenicillin) in the presence of their main degradation product (penicilloic acid) were developed. Forced acidic and basic degradation processes were applied at different time intervals. The formed degradation products were elucidated and quantified using surface enhanced Raman spectroscopy...
A comparative evaluation of antimicrobial effect of synthesized silver nanoparticles (AgNPs) of different shapes using different methods was performed. Spherical, triangular and hexagonal AgNPs with an average size of 40nm were chemically prepared and characterized by transmission electron microscope (TEM) and UV–visible spectroscopy. The antimicrobial effect of these different AgNPs against the gram...
A new, simple, efficient and rapid separation method based on ligandless-dispersive liquid–liquid microextraction (LL-DLLME) was developed for the preconcentration of rare earth elements (REEs) (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu) in natural water samples, followed by inductively coupled plasma mass spectrometry (ICP-MS) detection. Carbon tetrachloride and acetone were used as...
A sensitive and rapid reversed-phase ultra performance liquid chromatographic (UPLC) method for the simultaneous determination of tocopherols (α‐, β‐, γ‐, δ‐), tocotrienols (α‐, β‐, γ‐, δ‐), α‐tocopherol acetate and α‐tocopherol nicotinate is described. The separation was achieved using a Kinetex pentafluorophenyl (PFP) column (150×2.1mm, 2.6µm) with both photodiode array (PDA) and fluorescence (FL)...
Three planar miniaturized perchlorate membrane sensors (3×5mm 2 ) are prepared using a flexible Kaptan substrate coated with nitron-perchlorate (NT-ClO 4 ) [sensor 1], methylene blue-perchlorate (MB-ClO 4 ) [sensor II] and indium-porphyrin (In-Por) [sensor III] as electroactive materials in PVC membranes plasticized with 2-NPPE. Sensors I, II and III display near-Nernstian...
Novel uranyl selective polymeric membrane electrodes were prepared using three different low-cost and commercially available Cyanex extractants namely, bis(2,4,4-trimethylpentyl) phosphinic acid [L1], bis(2,4,4-trimethylpentyl) monothiophosphinic acid [L2] and bis(2,4,4-trimethylpentyl) dithiophosphinic acid [L3]. Optimization and performance characteristics of the developed Cyanex based polymer membrane...
This paper reports an improved way for performing highly reproducible surface enhanced Raman scattering of different analytes using an automated flow system. The method uses a confocal Raman microscope to prepare SERS active silver spots on the window of a flow cell by photo-reduction of silver nitrate in the presence of citrate. Placement of the flow cell on an automated x and y stages of the Raman...
Over the past years, passive sampling devices have been successfully used for the monitoring of various pollutants in water. The present work studied the uptake kinetics in surface water of ten polar pesticides and metabolites, using pharmaceutical POCIS samplers. The aim was to determine sampling rates from in-situ calibration and to compare results with those obtained earlier under laboratory conditions,...
Kinetic exclusion analysis (KinExA)-based immunosensors and enzyme-linked immunosorbent assays (ELISA) have been developed and validated for measurement of five different cancer markers in biological specimens. These markers were: 2′-deoxycytidine (dCyd), 8-hydroxy-2′-deoxyguanosine (8HdG), carbohydrate antigen (CA15-3), α-fetoprotein (AFP), and β-subunit of human chorionic gonadotropin (β-HCG). The...
We synthesized a series of 4-tert-butylcalix[4]arene nitriles (5–7) by the reaction of 4-tert-butylcalix[4]arene (1) with 4′-(6-bromohexyloxy)biphenyl-4-carbonitrile (2), 4′-(10-bromode-cyloxy)biphenyl-4-carbonitrile (3), and 4′-(12-bromododecyloxy)biphenyl-4-carbonitrile (4) and characterized their structures, respectively. The extraction abilities of newly synthesized 5–7 toward some selected heavy...
A new microextraction procedure termed multi-walled carbon nanotube-impregnated agarose film microextraction (MWCNT–AFME) has been developed. The method utilized multi-walled carbon nanotubes (MWCNTs) immobilized in agarose film to serve as adsorbent in solid phase microextraction (SPME). The film was prepared by mixing the MWCNTs in agarose solution and drying the mixture in oven. Extraction of selected...
Bare silica monoliths do not possess anion exchange sites hence they show low retention for anions. Moreover, bare silica monoliths show low retention in hydrophilic interaction liquid chromatography (HILIC). Coating the silica surface with cationic nanoparticles e.g. AS9-SC (latex A), AS12A (latex B) and DNApac (latex C) increases the thickness of the water layer on the Onyx silica monolith 8–10...
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