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A new electrochemical immunoassay strategy was proposed based on electrostatic interaction of natural polymers. The chitosan-entrapped carbon paste electrode (CCPE) was used as the base electrode, alginates as carriers for the reactants and horseradish peroxidase (HRP) as enzyme label in the immunoassay. The immunoassay was firstly carried out using the homogeneous competitive immune format and a...
A new strategy to construct amperometric immunosensor for Schistosoma japonicum antigen (SjAg) assay based on nano-size particulate gold (nano-Au) monolayer as sensing platform has been described. The nano-Au monolayer was obtained through chitosan-entrapped carbon paste electrode (CCPE). The high affinity of chitosan entrapped in CCPE for nano-Au associated with its amino groups facilitated the formation...
A procedure for fabricating an enzyme electrode has been described based on the effective immobilization of horseradish peroxidase (HRP) to a nano-scaled particulate gold (nano-Au) monolayer modified chitosan-entrapped carbon paste electrode (CCPE). The high affinity of chitosan entrapped in CCPE for nano-Au associated with its amino groups has been utilized to realize the use of nano-Au as an intermediator...
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