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In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate the genetic diversity among six Oratosquilla oratoria populations: Dalian (DL), Wafangdian (WF), Zhuanghe (ZH), Donggang (DG), Suizhong (SZ) in Liaoning province and Qingdao (QD) in Shandong province. 18 random primers for RAPD analyzing generated 256 bands, out of which 209 were polymorphic loci. The number of bands...
Random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeats (ISSR) markers were used to study the genetic diversity and relationships in three razor clam species (sinonovacula constricta, solen strictus and solen grandis). 14 random primers for RAPD analyzing generated 113 bands, out of which 105 (92.92%) were polymorphic loci and with an average of 8.1 bands per primer. Nine primers...
CYP17 (17α-hydroxylase/17, 20-lyase) has been viewed as a critical enzyme for the biosynthesis of gonadal and adrenal steroids in vertebrates. According to a reported CYP17 gene of Strongylocentrotus purpuratus gene from NCBI, three pairs of primers were designed to clone CYP17 gene of Strongylocentrotus nudus,using RT-PCR. Three fragments were assembled into on contig of 1569bp, including full CDS...
Embryonic stem cells (ESCs) hold promise either as an in vitro model recapitulating early embryonic development or as a renewable source of therapeutically useful cells. However, 2D culture system, which is still generally utilized in ESCs differentiation strategies, has greatly impeded our learning more information about ESCs proliferation/differentiation in situ. Here, a high aspect ratio vessel...
The major histocompatibility complex (MHC) is a gene cluster directly related to immune response and because of its important immunological functions, the structure and evolution history of MHC is of great interest to world's scientists. To elucidate the evolutionary history of MHC, the MHC class II antigen genes of 7 kinds of marine fish and 8 kinds of freshwater fish were selected. Then the phylogenetic...
In order to understand the phylogenetic relationships among Vibrio species more rationally , the hemolysin gene partial sequences of 4 Vibrio species (Vibrio alginolyticus, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio minius) were amplifyed and sequenced. We constructed two separate phylogenetic trees by using 16S rRNA and partial hemolysin gene sequences, respectively.The average similarities...
Phylogenetic analysis is widely used to determine evolution and function of gene, to identify the genus of algae based on gene sequences. Phylogenetic trees showing the evolutionary relationship of 20 Laminariales based on rbcL and 5.8S rRNA gene sequences were compared. The validity and the reliability of phylogenetic trees were studied in this paper. The results show that rbcL gene sequences are...
Phylogenetic analysis is widely used to determine evolution and function of gene, to identify the genus of bacteria based on gene sequences. Phylogenetic trees showing the evolutionary relationship of 32 Vibrios based on gyrB and 16S rRNA gene sequences are compared, the validity and the reliability of phylogenetic trees were studied in this paper. The result showed that gyrB gene sequences were reliable...
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