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Cis-regulatory elements are regions of DNA that regulate the expression of genes located on that same molecule of DNA. Though these elements are important for gene expression regulation, the functions of cis elements remain largely unknown. To explore the mechanisms of gene activation by short sequence enhancers, we examined the enhancer activity of short sequence DNA and its derived sequences using...
The presence of Alu repeats downregulates the expression of the green fluorescent protein (GFP) gene. We found that SV40PolyA (PolyA, 240 bp), in either orientation, eliminated the inhibition of GFP gene expression induced by Alu repeats when it was placed between the GFP gene and the Alu repeats. In this study, 4 different segments (each 60 bp) were amplified from antisense PolyA (PolyAas) by PCR,...
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