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Low frequency of targeted gene disruption in filamentous fungi has been attributed to the predominance of ectopic integration that is controlled by a non-homologous end-joining (NHEJ) DNA repair mechanism. It has been shown in a number of fungi that suppression of NHEJ by inactivating the yeast Ku70 or Ku80 homolog facilitates homologous recombination, thereby enhancing the frequency of gene targeting...
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