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Objectives To determine the origin of 15N-labeled phenylalanine in microbial metabolic flux analysis using 15N as a tracer, a method for measuring phenylalanine δ15N using HPLC coupled with elemental analysis-isotope ratio mass spectrometry (EA-IRMS) was developed. Results The original source of the 15N-labeled phenylalanine was determined using this new method that consists of three steps: optimization...
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