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The ATDC5 chondrogenic cell line is typically induced to differentiate by exposure to insulin at high concentration (10μg/ml, approximately 1600nM). Differentiation can also be induced by physiological concentrations of insulin-like growth factor-I (IGF-I). Unlike previous reports, we observed a stimulation of differentiation, as measured by collagen X expression and Alcian Blue staining for proteoglycan...
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