Wyniki wyszukiwania dla: V. Novotny

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Fractionation and characterization of sialyl linkage isomers of serum N‐glycans by CE–MS

Xiaomei Zhou, Woran Song, Milos V. Novotny, Stephen C. Jacobson

Journal of Separation Science > 45 > 17 > 3348 - 3361

Structural isomers of sialylated N‐glycans contribute to the diversity of the N‐glycome and to a range of biological functions. Sialyl linkage isomers can be readily distinguished by mass spectrometry with mass differences between α2,3‐ and α2,6‐linkages generated by a two‐step sialic acid linkage‐specific alkylamidation. To improve the identification of N‐glycans from complex mixtures, we added a...

artykuł

Mass‐dependent parameters of extensive air showers with anomalous longitudinal profiles

V. Novotný, D. Nosek, J. Blažek

Astronomische Nachrichten > 340 > 1-3 > 234 - 236

We investigate possibilities to determine the mass of a primary particle from anomalous longitudinal profiles of extensive air showers. Such profiles are predicted utilizing a Monte Carlo technique together with the contemporary high‐energy interaction models. The depth of shower maximum, which is commonly used for the mass composition analyses, is inspected and its performance to discriminate between...

artykuł

Microgradient separation technique for purification and fractionation of permethylated N‐glycans before mass spectrometric analyses

Pavel Rehulka, Martina Zahradnikova, Helena Rehulkova, Petra Dvorakova, więcej

Journal of Separation Science > 41 > 9 > 1973 - 1982

Analysis of N‐glycans released enzymatically from patients’ sera or other clinical samples may provide diagnostically and prognostically important information on human disease. Permethylation of these biomolecules simultaneously increases their hydrophobicity and substantially improves their detection parameters in the following mass spectrometric analyses. The overall procedure, from the glycan cleavage...

artykuł

Analytical Scheme Leading to Integrated High-Sensitivity Profiling of Glycosphingolipids Together with N- and O-Glycans from One Sample

John D. Benktander, Solomon T. Gizaw, Stefan Gaunitz, Milos V. Novotny

Journal of The American Society for Mass Spectrometry > 2018 > 29 > 6 > 1125-1137

Glycoconjugates are directly or indirectly involved in many biological processes. Due to their complex structures, the structural elucidation of glycans and the exploration of their role in biological systems have been challenging. Glycan pools generated through release from glycoprotein or glycolipid mixtures can often be very complex. For the sake of procedural simplicity, many glycan profiling...

artykuł

Peri‐operative allogeneic blood transfusion does not adversely affect oncological outcomes after radical cystectomy for urinary bladder cancer: a propensity score‐weighted European multicentre study

Malte W. Vetterlein, Philipp Gild, Luis A. Kluth, Thomas Seisen, więcej

BJU International > 121 > 1 > 101 - 110

Objectives To evaluate the effect of peri‐operative blood transfusion (PBT) on recurrence‐free survival, overall survival, cancer‐specific mortality and other‐cause mortality in patients undergoing radical cystectomy (RC), using a contemporary European multicentre cohort. Patients and Methods The Prospective Multicentre Radical Cystectomy Series (PROMETRICS) includes data on 679 patients who underwent...

artykuł

Contrast‐enhanced sonothrombolysis in acute ischemic stroke patients without intracranial large‐vessel occlusion

C. E. Kvistad, A. Nacu, V. Novotny, N. Logallo, więcej

Acta Neurologica Scandinavica > 137 > 2 > 256 - 261

Background Contrast‐enhanced sonothrombolysis (CEST) leads to a more rapid recanalization in acute ischemic stroke caused by intracranial large‐vessel occlusion (LVO). Animal studies have shown that CEST also may be safe and efficient in treating the ischemic microcirculation in the absence of LVO. The exact mechanism behind this treatment effect is not known. We aimed to assess safety and efficacy...

artykuł

Capillary electrophoresis–mass spectrometry for direct structural identification of serum N-glycans

Christa M. Snyder, Xiaomei Zhou, Jonathan A. Karty, Bryan R. Fonslow, więcej

Journal of Chromatography A > 2017 > 1523 > C > 127-139

Through direct coupling of capillary electrophoresis (CE) to mass spectrometry (MS) with a sheathless interface, we have identified 77 potential N-glycan structures derived from human serum. We confirmed the presence of N-glycans previously identified by indirect methods, e.g., electrophoretic mobility standards, obtained 31 new N-glycan structures not identified in our prior work, differentiated...

artykuł

Development of capillary liquid chromatography: A personal perspective

Milos V. Novotny

Journal of Chromatography A > 2017 > 1523 > C > 3-16

This is a historical account on the development of capillary LC from its beginning to the present day. The first investigations into the viability of capillary LC date back to the late 1970s, a decade after the pioneering efforts in HPLC. The drastically reduced column dimensions were required to counter the slow solute diffusion in liquids. There were numerous instrumental difficulties with sample...

artykuł

Songbird chemical signals reflect uropygial gland androgen sensitivity and predict aggression: implications for the role of the periphery in chemosignaling

Danielle J. Whittaker, Kimberly A. Rosvall, Samuel P. Slowinski, Helena A. Soini, więcej

Journal of Comparative Physiology A > 2018 > 204 > 1 > 5-15

Chemical signals can provide useful information to potential mates and rivals. The production mechanisms of these signals are poorly understood in birds, despite emerging evidence that volatile compounds from preen oil may serve as chemosignals. Steroid hormones, including testosterone (T), may influence the production of these signals, yet variation in circulating T only partly accounts for this...

artykuł

Spectral calibration of the fluorescence telescopes of the Pierre Auger Observatory

A. Aab, P. Abreu, M. Aglietta, I. Al Samarai, więcej

Astroparticle Physics > 2017 > 95 > C > 44-56

We present a novel method to measure precisely the relative spectral response of the fluorescence telescopes of the Pierre Auger Observatory. We used a portable light source based on a xenon flasher and a monochromator to measure the relative spectral efficiencies of eight telescopes in steps of 5 nm from 280 nm to 440 nm. Each point in a scan had approximately 2 nm FWHM out of the monochromator....

rozdział

The analysis of reliability of solder joints on SMD ceramic resistor arrays

V. Novotny, J. Skacel, I. Szendiuch, J. Sandera

2017 40th International Spring Seminar on Electronics Technology (ISSE) > 1 - 5

2017 40th International Spring Seminar on Electronics Technology (ISSE)

This paper deals with the area of solder joints reliability and narrower focus is on solder joints on SMD ceramic resistor arrays in configuration 8x0603. These SMD ceramic resistor arrays are soldered on 30 testing boards and every board includes two packages. Testing boards are exposed to influences of temperature changes in the temperature chamber. The range of temperature changes is from −25 °C...

artykuł

Speciation in a keystone plant genus is driven by elevation: a case study in New Guinean Ficus

S. T. Segar, M. Volf, J. Zima Jnr, B. Isua, więcej

Journal of Evolutionary Biology > 30 > 3 > 512 - 523

Much of the world's insect and plant biodiversity is found in tropical and subtropical ‘hotspots’, which often include long elevational gradients. These gradients may function as ‘diversity pumps’ and contribute to both regional and local species richness. Climactic conditions on such gradients often change rapidly along short vertical distances and may result in local adaptation and high levels of...

artykuł

Comparing oncological outcomes of laparoscopic versus open nephroureterectomy for the treatment of upper tract urothelial carcinoma: A propensity match analysis

M. Moschini, T. Seisen, M. Roupret, B. Foerster, więcej

European Urology Supplements > 2017 > 16 > 3 > e2017-e2018

artykuł

Trends of lymphadenectomy in upper tract urothelial carcinoma patients treated with radical nephroureterectomy: The impact of surgical technique

M. Moschini, T. Seisen, M. Roupret, P. Colin, więcej

European Urology Supplements > 2017 > 16 > 3 > e2021-e2022

artykuł

Acute cerebral infarcts in multiple arterial territories associated with cardioembolism

V. Novotny, L. Thomassen, U. Waje‐Andreassen, H. Naess

Acta Neurologica Scandinavica > 135 > 3 > 346 - 351

Objectives It is generally believed that cardioembolism is the main cause of multiple acute cerebral infarcts (MACI). However, there are surprisingly few DWI studies and results are conflicting. Based on a large prospective study we hypothesized that MACI are associated with cardioembolism. Materials and methods We studied 2697 patients with acute cerebral infarcts between February 2006 and October...

artykuł

Voltammetric determination of Aclonifen at a silver amalgam electrode in drinking and river water

V. Novotný, J. Barek

Ecological Chemistry and Engineering. S > 2017 > Vol. 24, nr 2 > 277--284

A method for the determination of pesticide Aclonifen (AC) in drinking and river water by differential pulse voltammetry (DPV) on a meniscus modified silver solid amalgam electrode (m-AgSAE) using solid phase extraction (SPE) as a cleanup and preconcentration procedure is described. The limit of detection (LOD) for direct DPV determination of AC in deionized water is 2.7·10^-8mol·dm^-3. LOD for DPV...

artykuł

Evidence for a mixed mass composition at the ‘ankle’ in the cosmic-ray spectrum

A. Aab, P. Abreu, M. Aglietta, E.J. Ahn, więcej

Physics Letters B > 2016 > 762 > C > 288-295

We report a first measurement for ultrahigh energy cosmic rays of the correlation between the depth of shower maximum and the signal in the water Cherenkov stations of air-showers registered simultaneously by the fluorescence and the surface detectors of the Pierre Auger Observatory. Such a correlation measurement is a unique feature of a hybrid air-shower observatory with sensitivity to both the...

artykuł

Evolutionary Interactions Between Visual and Chemical Signals: Chemosignals Compensate for the Loss of a Visual Signal in Male Sceloporus Lizards

Jake A. Pruett, J. Jaime Zúñiga-Vega, Stephanie M. Campos, Helena A. Soini, więcej

Journal of Chemical Ecology > 2016 > 42 > 11 > 1164-1174

Animals rely on multimodal signals to obtain information from conspecifics through alternative sensory systems, and the evolutionary loss of a signal in one modality may lead to compensation through increased use of signals in an alternative modality. We investigated associations between chemical signaling and evolutionary loss of abdominal color patches in males of four species (two plain-bellied...

artykuł

Urinary volatile compounds differ across reproductive phenotypes and following aggression in male Siberian hamsters

Nikki M. Rendon, Helena A. Soini, Melissa-Ann L. Scotti, Milos V. Novotny, więcej

Physiology & Behavior > 2016 > 164 > PA > 58-67

Chemical communication plays an integral role in social behavior by facilitating social encounters, allowing for the evaluation of social partners, defining territories and advertising information such as species and sex. Odors provide information about the social environment for rodents and other mammals; however, studies identifying chemical compounds and their functions have thus far focused primarily...

artykuł

Volatile organic compounds (VOCs) drive nutrient foraging in the clonal woodland strawberry, Fragaria vesca

Erica M. Waters, Helena A. Soini, Milos V. Novotny, Maxine A. Watson

Plant and Soil > 2016 > 407 > 1-2 > 261-274

Background and Aims It was previously demonstrated that stolons of Fragaria vesca respond to patches of varying nutrient quality; however, the mechanism of patch-detection remained unknown. Here we provide support for a process by which F. vesca perceives nutrient-rich patches, consistent with nutrient foraging prior to rooting. Methods Volatile organic compounds (VOCs) emitted from unsterilized...

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