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We cloned two genes from the salt-tolerant yeast Zygosaccharomyces rouxii: ZrSOD2 for the cell membrane Na + /H + -antiporter and ZrPMA1 for the cell membrane H + -ATPase. The products of these genes play cooperative roles in the salt-tolerance of Z. rouxii, and the function of the ZrPMA1 product is regulated at the transcription level. We constructed a yeast expression vector...
We examined the effects of heterologous expression of the open reading frames (ORF) of two genes on salt tolerance and glycerol production in a Saccharomyces cerevisiae strain deficient in glycerol synthesis (gpd1Δgpd2Δ). When the ORF of the Zygosaccharomyces rouxii glycerol 3-phosphate dehydrogenase gene (ZrGPD1) was expressed under the control of the GAL10 promoter, salt tolerance and glycerol production...
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