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The bphA1(2072)A2A3A4 gene cluster codes for a shuffled biphenyl dioxygenase holoenzyme with broad substrate specificity. The bphA1(2072) encoding the large subunit of an iron-sulfur protein is a hybrid gene generated by DNA shuffling using bphA1 of Pseudomonas pseudoalcaligenes KF707 and bphA1 of Burkholderia cepacia LB400. The bphA2 encoding the small subunit of an iron-sulfur protein, bphA3 encoding...
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