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To develop a simple and sensitive LC–MS/MS procedure for quantification of serum 25-OH-vitamin D 3 (25-OH-D 3 ), 25-OH-vitamin D 2 (25-OH-D 2 ), and their C3-epimers.Serum 25-OH-vitamin D metabolites were extracted with MTBE and quantified by LC–MS/MS. Commercially available calibrators and QC materials were employed. The ion-transition 401.2→365.2 was monitored for...
With an ever-increasing clinical interest in vitamin D insufficiency, numerous automated immunoassays, protein binding assays, and in-house LC-MS/MS methods are being developed for the quantification of 25-hydroxyvitamin D 3 (25(OH)D 3 ). Recently, LC-MS/MS methods have identified an epimeric form of 25(OH)D 3 that has been shown to contribute significantly to 25(OH)D 3...
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