Search results for: Dan H. Barouch

Items from 1 to 20 out of 47 results

article

Sustained maternal antibody and cellular immune responses in pregnant women infected with Zika virus and mother to infant transfer of Zika‐specific antibodies

Ai‐ris Y. Collier, Erica N. Borducchi, Abishek Chandrashekar, Edward Moseley, more

American Journal of Reproductive Immunology > 84 > 4 > n/a - n/a

Problem Evaluation of Zika virus (ZIKV)‐specific humoral and cellular immune response in pregnant women exposed to ZIKV. Method of Study In this observational, prospective cohort study, we recruited pregnant women presenting for prenatal ultrasound for ZIKV exposure at a single academic teaching hospital in Boston, MA from November 2016 to December 2018. We collected blood, urine, and cervicovaginal...

article

A microbial expression system for high-level production of scFv HIV-neutralizing antibody fragments in Escherichia coli

Marloes L. C. Petrus, Lukas A. Kiefer, Pranav Puri, Evert Heemskerk, more

Applied Microbiology and Biotechnology > 2019 > 103 > 21-22 > 8875-8888

Monoclonal antibodies (mABs) are of great biopharmaceutical importance for the diagnosis and treatment of diseases. However, their production in mammalian expression hosts usually requires extensive production times and is expensive. Escherichia coli has become a new platform for production of functional small antibody fragment variants. In this study, we have used a rhamnose-inducible expression...

article

T‐cell subset differentiation and antibody responses following antiretroviral therapy during simian immunodeficiency virus infection

Mitra Bhattacharyya, James B. Whitney, Michael Seaman, Dan H. Barouch, more

Immunology > 155 > 4 > 458 - 466

Antiretroviral therapy (ART) for the treatment of human immunodeficiency virus (HIV) infection represents a major breakthrough in the treatment of HIV/acquired immune‐deficiency syndrome. However, it remains unclear how ART influences virus‐specific immune responses and understanding this is important for developing novel cure and eradication interventions for HIV‐1. In the present study, we evaluate...

article

Evaluation of a mosaic HIV-1 vaccine in a multicentre, randomised, double-blind, placebo-controlled, phase 1/2a clinical trial (APPROACH) and in rhesus monkeys (NHP 13-19)

Dan H Barouch, Frank L Tomaka, Frank Wegmann, Daniel J Stieh, more

The Lancet > 2018 > 392 > 10143 > 232-243

More than 1·8 million new cases of HIV-1 infection were diagnosed worldwide in 2016. No licensed prophylactic HIV-1 vaccine exists. A major limitation to date has been the lack of direct comparability between clinical trials and preclinical studies. We aimed to evaluate mosaic adenovirus serotype 26 (Ad26)-based HIV-1 vaccine candidates in parallel studies in humans and rhesus monkeys to define the...

article

Fetal Neuropathology in Zika Virus-Infected Pregnant Female Rhesus Monkeys

Amanda J. Martinot, Peter Abbink, Onur Afacan, Anna K. Prohl, more

Cell > 2018 > 173 > 5 > 1111-1122.e10

The development of interventions to prevent congenital Zika syndrome (CZS) has been limited by the lack of an established nonhuman primate model. Here we show that infection of female rhesus monkeys early in pregnancy with Zika virus (ZIKV) recapitulates many features of CZS in humans. We infected 9 pregnant monkeys with ZIKV, 6 early in pregnancy (weeks 6–7 of gestation) and 3 later in pregnancy...

article

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells

Mauro Gaya, Patricia Barral, Marianne Burbage, Shweta Aggarwal, more

Cell > 2018 > 172 > 3 > 517-533.e20

B cells constitute an essential line of defense from pathogenic infections through the generation of class-switched antibody-secreting cells (ASCs) in germinal centers. Although this process is known to be regulated by follicular helper T (TfH) cells, the mechanism by which B cells initially seed germinal center reactions remains elusive. We found that NKT cells, a population of innate-like T lymphocytes,...

article

308: Altered decidua regulatory T cell proportion and phenotype in preeclampsia

Ai-ris Y. Collier, Jinyan Liu, Dan H. Barouch

American Journal of Obstetrics and Gynecology > 2018 > 218 > 1(S) > S195

article

HIV-1 Latency by Transition

Boris Julg, Dan H. Barouch

Immunity > 2017 > 47 > 4 > 611-612

The latent HIV-1 reservoir represents the major barrier for the development of an HIV-1 cure. In this issue of Immunity, Shan et al. (2017) highlight that effector-to-memory transitioning (EMT) CD4⁺ T cells are particularly permissive for the establishment of latent HIV-1 infection.

article

Regulation of CD4 T cells and their effects on immunopathological inflammation following viral infection

Mitra Bhattacharyya, Patrick Madden, Nathan Henning, Shana Gregory, more

Immunology > 152 > 2 > 328 - 343

CD4 T cells help immune responses, but knowledge of how memory CD4 T cells are regulated and how they regulate adaptive immune responses and induce immunopathology is limited. Using adoptive transfer of virus‐specific CD4 T cells, we show that naive CD4 T cells undergo substantial expansion following infection, but can induce lethal T helper type 1‐driven inflammation. In contrast, memory CD4 T cells...

article

Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches

Matthias Pauthner, Colin Havenar-Daughton, Devin Sok, Joseph P. Nkolola, more

Immunity > 2017 > 46 > 6 > 1073-1088.e6

The development of stabilized recombinant HIV envelope trimers that mimic the virion surface molecule has increased enthusiasm for a neutralizing antibody (nAb)-based HIV vaccine. However, there is limited experience with recombinant trimers as immunogens in nonhuman primates, which are typically used as a model for humans. Here, we tested multiple immunogens and immunization strategies head-to-head...

article

Zika Virus Persistence in the Central Nervous System and Lymph Nodes of Rhesus Monkeys

Malika Aid, Peter Abbink, Rafael A. Larocca, Michael Boyd, more

Cell > 2017 > 169 > 4 > 610-620.e14

Zika virus (ZIKV) is associated with severe neuropathology in neonates as well as Guillain-Barré syndrome and other neurologic disorders in adults. Prolonged viral shedding has been reported in semen, suggesting the presence of anatomic viral reservoirs. Here we show that ZIKV can persist in cerebrospinal fluid (CSF) and lymph nodes (LN) of infected rhesus monkeys for weeks after virus has been cleared...

article

Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles

Eric P. Brown, Karen G. Dowell, Austin W. Boesch, Erica Normandin, more

Journal of Immunological Methods > 2017 > 443 > C > 33-44

Antibodies are widely considered to be a frequent primary and often mechanistic correlate of protection of approved vaccines; thus evaluating the antibody response is of critical importance in attempting to understand and predict the efficacy of novel vaccine candidates. Historically, antibody responses have been analyzed by determining the titer of the humoral response using measurements such as...

article

Statoviruses, A novel taxon of RNA viruses present in the gastrointestinal tracts of diverse mammals

Andrew B. Janowski, Siddharth R. Krishnamurthy, Efrem S. Lim, Guoyan Zhao, more

Virology > 2017 > 504 > C > 36-44

Next-generation sequencing has expanded our understanding of the viral populations that constitute the mammalian virome. We describe a novel taxon of viruses named Statoviruses, for Stool associated Tombus-like viruses, present in multiple metagenomic datasets. These viruses define a novel clade that is phylogenetically related to the RNA virus families Tombusviridae and Flaviviridae. Five distinct...

article

VirusSeeker, a computational pipeline for virus discovery and virome composition analysis

Guoyan Zhao, Guang Wu, Efrem S. Lim, Lindsay Droit, more

Virology > 2017 > 503 > C > 21-30

The advent of Next Generation Sequencing (NGS) has vastly increased our ability to discover novel viruses and to systematically define the spectrum of viruses present in a given specimen. Such studies have led to the discovery of novel viral pathogens as well as broader associations of the virome with diverse diseases including inflammatory bowel disease, severe acute malnutrition and HIV/AIDS. Critical...

article

Vaccine Elicitation of High Mannose-Dependent Neutralizing Antibodies against the V3-Glycan Broadly Neutralizing Epitope in Nonhuman Primates

Kevin O. Saunders, Nathan I. Nicely, Kevin Wiehe, Mattia Bonsignori, more

Cell Reports > 2017 > 18 > 9 > 2175-2188

Induction of broadly neutralizing antibodies (bnAbs) that target HIV-1 envelope (Env) is a goal of HIV-1 vaccine development. A bnAb target is the Env third variable loop (V3)-glycan site. To determine whether immunization could induce antibodies to the V3-glycan bnAb binding site, we repetitively immunized macaques over a 4-year period with an Env expressing V3-high mannose glycans. Env immunizations...

article

Prospects for a Zika Virus Vaccine

Dan H. Barouch, Stephen J. Thomas, Nelson L. Michael

Immunity > 2017 > 46 > 2 > 176-182

A recent unprecedented outbreak of Zika virus (ZIKV) in the Americas has been associated with microcephaly and other congenital malformations in infants as well as Guillain-Barre syndrome in adults. The development of a safe and effective ZIKV vaccine is therefore an urgent global health priority. Promising data from preclinical vaccine studies in mice and monkeys suggest that an effective vaccine...

article

Systems serology for evaluation of HIV vaccine trials

Margaret E. Ackerman, Dan H. Barouch, Galit Alter

Immunological Reviews > 275 > 1 > 262 - 270

The scale and scope of the global epidemic, coupled to challenges with traditional vaccine development approaches, point toward a need for novel methodologies for HIV vaccine research. While the development of vaccines able to induce broadly neutralizing antibodies remains the ultimate goal, to date, vaccines continue to fail to induce these rare humoral immune responses. Conversely, growing evidence...

article

Development of novel replication-defective lymphocytic choriomeningitis virus vectors expressing SIV antigens

Pablo Penaloza MacMaster, Jennifer L. Shields, Quazim A. Alayo, Crystal Cabral, more

Vaccine > 2017 > 35 > 1 > 1-9

An important focus in vaccine research is the design of vaccine vectors with low seroprevalence and high immunogenicity. Replication-incompetent lymphocytic choriomeningitis virus (rLCMV) vectors do not elicit vector-neutralizing antibody responses, and homologous prime-boost regimens with rLCMV vectors induce boostable and protective T cell responses to model antigens in mice. However, cellular and...

article

Inhibitory receptor expression on memory CD8 T cells following Ad vector immunization

Pablo Penaloza-MacMaster, Quazim A. Alayo, Joshua Ra, Nicholas M. Provine, more

Vaccine > 2016 > 34 > 41 > 4955-4963

T cells are an important component of immune responses, and their function is influenced by their expression of inhibitory receptors. Immunization with alternative serotype adenovirus (Ad) vectors induces highly functional T cell responses with lower programmed cell death 1 (PD-1) expression and increased boostability relative to Ad5 vectors. However, a detailed phenotypic characterization of other...

article

The sooner the better: innate immunity as a path toward the HIV cure

Jeffrey Tomalka, Khader Ghneim, Sanghamitra Bhattacharyya, Malika Aid, more

Current Opinion in Virology > 2016 > 19 > C > 85-91

To combat the diverse pathogens that infect humans, the immune system has evolved complex and diverse transcriptional signatures, which drive differential cellular and humoral responses. These signatures are induced by immune receptor sensing of pathogens and by cytokines produced at the earliest onset of infection. The specific nature of immune activation is as critical to pathogen clearance as the...

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INFONA - science communication portal

Search results for: Dan H. Barouch

Sustained maternal antibody and cellular immune responses in pregnant women infected with Zika virus and mother to infant transfer of Zika‐specific antibodies

A microbial expression system for high-level production of scFv HIV-neutralizing antibody fragments in Escherichia coli

T‐cell subset differentiation and antibody responses following antiretroviral therapy during simian immunodeficiency virus infection

Evaluation of a mosaic HIV-1 vaccine in a multicentre, randomised, double-blind, placebo-controlled, phase 1/2a clinical trial (APPROACH) and in rhesus monkeys (NHP 13-19)

Fetal Neuropathology in Zika Virus-Infected Pregnant Female Rhesus Monkeys

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells

308: Altered decidua regulatory T cell proportion and phenotype in preeclampsia

HIV-1 Latency by Transition

Regulation of CD4 T cells and their effects on immunopathological inflammation following viral infection

Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches

Zika Virus Persistence in the Central Nervous System and Lymph Nodes of Rhesus Monkeys

Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles

Statoviruses, A novel taxon of RNA viruses present in the gastrointestinal tracts of diverse mammals

VirusSeeker, a computational pipeline for virus discovery and virome composition analysis

Vaccine Elicitation of High Mannose-Dependent Neutralizing Antibodies against the V3-Glycan Broadly Neutralizing Epitope in Nonhuman Primates

Prospects for a Zika Virus Vaccine

Systems serology for evaluation of HIV vaccine trials

Development of novel replication-defective lymphocytic choriomeningitis virus vectors expressing SIV antigens

Inhibitory receptor expression on memory CD8 T cells following Ad vector immunization

The sooner the better: innate immunity as a path toward the HIV cure

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