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The widespread replicons of repABC and repC families from α-proteobacteria share high similarity in their replication initiator proteins (RepC). Here we describe the minimal region required for stable replication of a member of the repC family, the low copy-number plasmid pRmeGR4a from Sinorizobium meliloti GR4. This region contains only two genes: one encoding the initiator protein RepC (46.8kDa)...
The effects of hydrostatic high pressure, microwave irradiation, and conventional heating on hydrolysis of bovine β-lactoglobulin AB by pronase and α-chymotrypsin were studied. The high pressure treatments were performed between 100 and 300 MPa, the microwave irradiation was applied at different power conditions, pronase (30 W) and α-chymotrypsin (15 W). All enzymatic reactions were carried out during...
In this paper, we report the identification of replication/partition regions of plasmid p42a and p42b of Rhizobium etli CFN42. Sequence analysis reveals that both replication/partition regions belong to the repABC family. Phylogenetic analysis of all the complete repABC replication/partition regions reported to date, shows that repABC plasmids coexisting in the same strain arose most likely by lateral...
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