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To test the toxic effects of a diterpene lactone, diosbulbin-D, isolated from Dioscorea bulbifera L. on hepatocytes. Using the MTT assay to test the effect of diosbulbin-D on hepatocytes. Selected intracellular enzymes release levels by diosbulbin-D were determined to further validate the toxic effect on hepatocytes. Furthermore, the level of intracellular glutathione and the induction of reactive...
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