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In flowering plants, pollen germinates to deliver sperm cells to the egg and central cells during double fertilization. In a bicellular pollen species, sperm cells are formed as the pollen tube grows. However, the process of pollen tube growth is difficult to observe as it proceeds in inside the pistil. Here, an in vitro liquid pollen culture system was applied to pollen of Petunia cultivars to analyze...
Pollen tubes of Cyrtanthus mackenii, a species with bicellular pollen, were cultured in vitro to investigate nuclear phase changes during generative cell division and male germ unit (MGU) formation, using flow cytometric analysis. Results revealed that sperm cells were formed after 12 h of culture. During sperm maturation, the nuclei of sperm cells were not associated with the vegetative nucleus (unassociated...
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