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We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O 2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K 0.5 of 15 μM. The stimulatory effect of NPPB also O 2 consumption, like that...
The mechanisms underlying cytoplasmic pH (pH i ) regulation in elicited rat peritoneal macrophages were investigated by electronic sizing and fluorescence determinations. Acid-loaded cells rapidly regained normal pH i by means of an amiloride-sensitive Na + /H + exchange. When stimulated by 12-O-tetradecanoyl phorbol 13-acetate, macrophages displayed a biphasic pH ...
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