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Microsecond-duration electric pulses are widely used for research and therapeutic purposes. The most commonly used device for in vitro exposure to electric pulses is a standard electroporation cuvette. The use of nanosecond-duration electric pulses [nanosecond pulsed electromagnetic fields (nsPEFs) or nanopulses] is an emerging technology. For microsecond pulses, some dedicated generators with voltage...
Intense (several MV/m) nanosecond duration electric pulses can induce damages on cells external membrane which can be detected by direct cell killing or by the uptake of non permeant molecules. We detail here the methodology to carry out those experiments, drawing the attention to exposure artifacts that demonstrate the importance of the implementation of a metrology specific to the ultrashort pulses.
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