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Staphylococcal nuclease (SNase) is a well-established model for protein folding studies. Its three-dimensional structure has been determined. The enzyme, Ca 2+ , and DNA or RNA substrate form a ternary complex. Glycine 20 is the second position of the first β-turn of SNase, which may serve as the folding initiation site for the SNase polypeptide. To study the role of Gly20 in the conformational...
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