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Dengue virus type 2 (DEN-2), a member of the Flaviviridae family, has a positive-strand RNA genome, 10 723 nucleotides (nt) in length and encoding a single polyprotein precursor consisting of 3391 amino acids (aa). In order to construct a full-length cDNA clone, the viral genome was cloned into 5′ (nt 1-2203 under the control of the T7 promoter (p T7 )) and 3′ (nt 2203–10723) constructs. A...
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