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Background: The human IL4 gene, has been shown to express the alternatively spliced messenger (m)RNA IL-4δ2. IL-4δ2 is missing the entire sequence from exon 2 and has been identified as an IL-4 receptor antagonist. Objective: We sought to distinguish IL-4 and IL-4δ2 mRNA in respiratory tract tissue for the first time. Methods: A novel competitive PCR assay was established with primers designed on...
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